Hydrogen donor specificity of cobamide-dependent ribonucleotide reductase and allosteric regulation of substrate specificity.
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چکیده
منابع مشابه
Regulation of Cobamide-dependent Ribonucleotide Reductase by Allosteric Effecters and Divalent Cations*
Factors regulating the rate behavior and substrate specificity of purified cobamide-dependent ribonucleotide reductase of Lacfobacillus leichmannii have been examined in detail. Reduction of each of the four common ribonucleoside triphosphates (@dine triphosphate, uridine triphosphate, adenosine triphosphate, and guanosine triphosphate) is maximally stimulated by a different deoxyribonucleoside...
متن کاملRegulation of cobamide-dependent ribonucleotide reductase by allosteric effectors and divalent cations.
Factors regulating the rate behavior and substrate specificity of purified cobamide-dependent ribonucleotide reductase of Lacfobacillus leichmannii have been examined in detail. Reduction of each of the four common ribonucleoside triphosphates (@dine triphosphate, uridine triphosphate, adenosine triphosphate, and guanosine triphosphate) is maximally stimulated by a different deoxyribonucleoside...
متن کاملMolecular basis for allosteric specificity regulation in class Ia ribonucleotide reductase from Escherichia coli
Ribonucleotide reductase (RNR) converts ribonucleotides to deoxyribonucleotides, a reaction that is essential for DNA biosynthesis and repair. This enzyme is responsible for reducing all four ribonucleotide substrates, with specificity regulated by the binding of an effector to a distal allosteric site. In all characterized RNRs, the binding of effector dATP alters the active site to select for...
متن کاملThe mechanism of action of cobamide coenzyme in the ribonucleotide reductase reaction.
When highly purified cobamide-dependent ribonucleotide reductase from Lacfobacillus leichmannii is incubated with synthetically prepared 5,6-dimethylbenzimidazolylcobamide 5’-deoxyadenosyl coenzyme containing tritium attached to carbon atom 5’ of the deoxyadenosyl moiety (DBCC-5’-3H), tritium is transferred from DBCC-5’-3H to Hz0 in a reaction requiring substrate, enzyme, and dithiol reductant....
متن کاملRibonucleotide reductases: the evolution of allosteric regulation.
Ribonucleotide reductases catalyze in all living organisms the production of the deoxyribonucleotides required for DNA replication and repair. Their appearance during evolution was a prerequisite for the transition from the "RNA world," where RNA sufficed for both catalysis and information transfer, to today's situation where life depends on the interplay among DNA, RNA, and protein. Three clas...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 241 9 شماره
صفحات -
تاریخ انتشار 1966